Labor is the largest asset in the workplace, and the management of occupational safety and health in their working environment and the protection of their physical and mental health are extremely important. From the perspective of occupational health and safety, fatigue must be managed because it has significant short-term and long-term impacts. Unlike traditional systems that only monitor heart rate, the labor safety physiological monitoring clothing system developed by the Textile Institute can simultaneously capture six types of fatigue biosensing devices, and use Bluetooth technology to conduct real-time monitoring of multiple data through mobile apps to prevent the occurrence of occupational accidents.